Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of Pantoea ananatis causing grain discolouration and leaf blight of rice in Russia.

Abstract

Since August 2011, leaf blight of rice plants (Oryza sativa) and grain discoloration on harvested seeds were observed in commercial fields in Primorsky Krai (region) in the Far East of Russia. Initially, light, rusty, water-soaked lesions, which later turned brown, appeared on the plant lemma or palea. Many immature and lighter grains, some with bacterial yellow ooze were observed on panicles at harvest. For bacterial isolation, samples of 400 seeds of several locally produced seed stocks were surface sterilised in 0.7% sodium hypochlorite, incubated for 30 minutes at 25°C, crushed and passed through a coarse filter. A sample of seed extract was placed onto yeast dextrose carbonate agar in Petri dishes in duplicates and incubated at 37±2°C for two days. Yellow colonies were sub-cultured and used for further identification. Based on biochemical, physiological, molecular (16S rRNA sequencing) and pathogenicity tests, the pathogen was identified as P. ananatis. This is thought to be the first report of P. ananatis causing a disease of rice in Russia, although a similar disease has been reported in nearby regions of China.