First report of natural occurence of Papaya leaf crumple virus on soyabean in India.
In Uttar Pradesh, India, symptoms of leaf crumple disease at Lalitpur and yellow mosaic disease at Lucknow were noticed on a number of soyabean plants in 2013. The naturally infected soyabean plants exhibited yellow mosaic on leaves and leaf crumpling and distortion symptoms. Severely infected plants were stunted and bore less number of flowers and pods compared to the healthy ones. Based on leaf crumple and yellow mosaic symptoms, begomovirus infection was suspected. Twenty-two leaf samples of infected soyabean plants were collected from each location and total DNA was isolated using a plant genomic DNA isolation kit. Based on the results of PCR assay and BLAST and phylogenetic analyses of the nucleotide sequences, two causal agents were identified, namely Mungbean yellow mosaic India virus (MYMIV) and Papaya leaf crumple virus (PaLCrV). A literature survey revealed the natural occurrence of MYMIV, Tomato leaf curl Karnatka virus and Cotton leaf curl Kokhran virus on soyabean in India. The existence of PaLCrV is reported on Carica papaya, Solanum nigrum and Andrographis paniculata from India. However, this is thought to be the first report of natural occurrence of PaLCrV in soyabean from India.