Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of Ageratum enation virus and Ageratum leaf curl betasatellite infecting Calendula officinalis in India.

Abstract

In January 2014, yellow vein net disease was observed on a number of C. officinalis plants growing in a garden in Lucknow, Uttar Pradesh, India. The severely infected C. officinalis plants exhibited yellow vein net symptoms on leaves and leaf deformation compared to healthy plants. The severely infected plants remained stunted and bore smaller sized and fewer flowers. Based on the typical yellow net symptoms described earlier on C. officinalis plants caused by a begomovirus, begomovirus infection was suspected. PCR amplification and DNA sequence analysis were carried out and the results revealed the causal agents as Ageratum enation virus (AEV) and Ageratum leaf curl betasatellite (ALCuB), which was confirmed by phylogenetic analysis and pathogenicity tests. Reports of natural occurrence of Cucumber mosaic virus, an unidentified begomovirus from India and Bidens mottle virus from Taiwan on C. officinalis are known. This is thought to be the first report of AEV and ALCuB infecting C. officinalis in India.