Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Effects of the Bax channel on cantharidin induced apoptosis of Spodoptera frugiperda Sf9 cells.

Abstract

Objectives: To investigate the role of the Bax channel on cantharidin-induced cell apoptosis in lepidopteran insects. Methods: Bax channel blocker was used to determine the roles of mitochondrial membrane potential, succinate dehydrogenase activity in mitochondria, and cell morphology, on apoptosis of Sf9 cells derived from fall the armyworm, Spodoptera frugiperda (J.E. Smith) exposed to cantharidin stress. Results: The results show that Bax channel blocker can delay the decrease of mitochondrial membrane potential and reduce changes in cell morphology, but fail to stop the decline of succinate dehydrogenase activity, in Sf9 cells after treatment with cantharidin. Conclusion: Bax related channels play a role in cantharidin-induced changes in mitochondrial membrane potential and destruction of cell morphology. However, the Bax channel does not appear to play a role in the suppression of enzyme activity in mitochondria after cantharidin treatment.