Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of Tomato leaf curl Sinaloa virus infecting tomato crops in Panama.

Abstract

In April 2011 and September 2012, virus-like symptoms were observed in open field- and greenhouse-grown tomato crops in Chiriquí, the westernmost province of Panama. Samples from symptom-bearing plants (127 in all) were collected and tested for the presence of begomoviruses by polymerase chain reaction (PCR) assays with sets of degenerated primers designed to amplify parts of the DNA-A and DNA-B components. Products of the expected sizes, obtained with both DNA-A- and DNA-B-specific primers for 49 samples, suggested infection with New World bipartite begomoviruses. This corresponds to an incidence of 26% (8 plants) in open field, and 43% (41 plants) in greenhouse crops. Primers specific for 10 tomato-infecting begomoviruses found in Central America were used to typify the PCR-positive samples. This analysis revealed Potato yellow mosaic Panama virus (PYMPV) or Tomato leaf curl Sinaloa virus (ToLCSiV) in 44 (90%) or 40 (82%) of the samples, respectively. All contained at least one virus, the majority (i.e. 35) indeed both, with no indication of the other viruses tested. These results were further supported by BLAST analysis of the PCR products' sequences. This is thought to be the first detection of ToLCSiV in Panama, first report of PYMPV in Panama's western highlands, and the first ever report of PYMPV/ToLCSiV mixed infection.