Culture filtrate of Pleurotus ostreatus isolate Poa3 effect on egg mass hatching and juvenile 2 of Meloidogyne incognita and its potential for biological control.
A possible alternative to chemical nematicides is the use of biological control agents for the management of root-knot nematode. This research was conducted from 2006 to 2008. One Pleurotus ostreatus isolated from Nakhon Pathom locations in Thailand, was evaluated for their in vitro antagonistic effects against egg masses and the juveniles 2 (J2) stage of root knot nematode, Meloidogyne incognita. Toxic droplets water agar tests demonstrated that P. ostreatus isolate Poa3 gave significantly higher colonized egg masses and parasitized hatching J2. Filtrates of the tested Poa3 grown in 1% malt extract broth were toxic to the infected egg masses and J2. The results showed that the culture filtrate of Poa3 reduced egg hatching and paralyzed juveniles of M. incognita after inoculation. The egg masses on tomato roots, the density of J2 and the number of galls were reduced in screen house bioassays of tomato plants parasitized by M. incognita.