The causative of leaf spot on carob: isolation and identification.
The main objective of the present study was to isolation and identification of the cause carob leaves spots. In this study, a total of 4 fungal isolates (referred as A1, A2 and A3) were obtained from symptomatic leaves collected from carob trees distribution in El-Beida city and its suburbs. The pathogen associated with the disease was identified as Alternaria alternata (Fr.) Keissl. All the isolates produced olive-brown to medium-brown conidia with obclavate shape, but the isolates varied in size (length) of conidia, the number of cells per conidium, rate of growth and sporulation on media. Koch's postulate was verified by inoculating healthy leaves. This was the first report of leaf spot disease of carob caused by Alternaria alternata in Libya.