Biological and molecular detection of Citrus psorosis virus in the Northwest region of Morocco.
Psorosis is a well-known citrus disease causing serious losses in many countries, particularly in the Mediterranean basin. Psorosis-like bark scaling is frequently observed on trunks and branches of sweet orange, mandarin and grapefruit trees in Morocco. To determine if these symptoms are associated with the presence of Citrus psorosis virus (CPsV), RT-PCR was performed on 24 citrus trees from the Gharb, the Northwest region in Morocco and association of the observed symptoms with psorosis disease was examined by biological indexing. Detection of CPsV by RT-PCR was not always associated with observation of psorosis-like symptoms in field trees or in graft inoculated indicator plants, indicating that psorosis-like symptoms also may be induced by pathogens other than CPsV. This is the first report on a psorosis survey in Morocco based on biological indexing and molecular detection of CPsV.