Identification and characterisation of Sclerotinia homoeoparpa causing leaf blight in 4 warm-season turfgrass species.
This paper reports on field disease surveys carried out at several golf courses and college lawns in Hainan Province from 2011 to 2013. A serious disease similar to Dollar Spot was observed which affected 4 common warm-season turfgrass species. Based on comprehensive analyses including Koch's postulates tests, the identification of morphological characteristics and rDNA-ITS sequencing, the pathogen causing Dollar Spot disease in Hainan province was identified as Sclerotinia homoeocarpa. To our knowledge, this is the first report that S. homoeocarpa can cause Dollar Spot in Burmudagrass and Carpetgrass in China. Biological characteristics analysis showed that S. homoeocarpa grew well in conditions where the temperature ranged from 25-30°C and pH from 5-7. The optimum growth conditions were 28°C and pH 5. Hyphallost their viability in agar plugs that were incubated at 55°C for 10 minutes. Light promoted mycelium growth and all the tested carbon and nitrogen sources were utilized by S. homoeocarpa. Soluble starch had higher usage efficiency for mycelium growth than the other carbon sources, whereas D-mannitol and D-arabinose had lower usage efficiency. Ammonium nitrate and ammonium dihydrogen phosphate had higher usage efficiency for mycelium growth than the other nitrogen sources, whereas carbamide had lower usage efficiency.