Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Construction and identification of cDNA expression library from larva midgut of Hyphantria cunea.

Abstract

Hyphantria cunea is an important invasive pest, and its peritrophic matrix protein is a potential target for biological control of pests. The cDNA expression library from larva midgut of H. cunea was constructed by total RNA extraction, mRNA purification and reverse transcription cDNA synthesis methods. The primary library titre of 4.08×105 pfu/ml was obtained and the recombination efficiency was 95.7%. The length of inserted fragments varied from 0.8 to 2.3 kb with an average length of 1.77 kb. The cDNA library was amplified and stocked, and the titre was estimated as 3.92×108 pfu/ml. This high-quality cDNA library provided a reliable theoretical basis for cloning the peritrophic matrix protein gene from larvae of H. cunea and developing environmentally-friendly microbial insecticides.