Association of 'Candidatus Phytoplasma cynodontis' with the yellow leaf disease of ivy gourd in Thailand.
Ivy gourd (Coccinia grandis) is a small climbing perennial plant in the family Cucurbitaceae. The disease symptom of yellow leaves was observed frequently on ivy gourd in Hatyai, Songkhla, Thailand, during 2013. Infected tissue samples were assayed by nested PCR with the universal primer pair P1/P7, followed by R16F2n/R2 to amplify the 16S rDNA fragment. The approximately 1.2 kb PCR products that were observed in symptomatic leaves had the highest nucleotide sequence similarity (99%) with the 16SrXIV group phytoplasmas. To the author's knowledge, this is the first report of ivy gourd as a host of 'Candidatus Phytoplasma cynodontis' in Thailand or elsewhere.