A new alphasatellite molecule associated with Ageratum yellow vein China virus in the Philippines.
From Synedrella nodiflora plants with leaf curling, vein swelling and enation symptoms on Samal Island, the Philippines, a begomoviral DNA-A and its associated alphasatellite molecule were cloned and sequenced. The begomovirus was identified as an isolate of Ageratum yellow vein China virus (AYVCNV) with 91% nucleotide sequence identity to AYVCNV-[P157] (EU487045), while the alphasatellite molecule was most closely related to tobacco curly shoot alphasatellite-Y99 (TbCSA-Y99, AJ579347) with 74.5% nucleotide sequence identity. The satellite molecule has the typical features of alphasatellites, with a single gene in the virion sense, an A-rich region and a 33-bp predicted stem-loop structure. According to the proposed species demarcation threshold of alphasatellites (83% nucleotide sequence identity), the alphasatellite molecule represents a new species, herein named 'Ageratum yellow vein China alphasatellite' (KF785752).