Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

A new alphasatellite molecule associated with Ageratum yellow vein China virus in the Philippines.

Abstract

From Synedrella nodiflora plants with leaf curling, vein swelling and enation symptoms on Samal Island, the Philippines, a begomoviral DNA-A and its associated alphasatellite molecule were cloned and sequenced. The begomovirus was identified as an isolate of Ageratum yellow vein China virus (AYVCNV) with 91% nucleotide sequence identity to AYVCNV-[P157] (EU487045), while the alphasatellite molecule was most closely related to tobacco curly shoot alphasatellite-Y99 (TbCSA-Y99, AJ579347) with 74.5% nucleotide sequence identity. The satellite molecule has the typical features of alphasatellites, with a single gene in the virion sense, an A-rich region and a 33-bp predicted stem-loop structure. According to the proposed species demarcation threshold of alphasatellites (83% nucleotide sequence identity), the alphasatellite molecule represents a new species, herein named 'Ageratum yellow vein China alphasatellite' (KF785752).