First report of Fusarium oxysporum f. sp. lycopersici race 3 and F. oxysporum f. sp. radicis-lycopersici in tomatoes in the Azapa Valley of Chile.
Tomato is an important crop in the Azapa Valley in northern Chile, with approximately 600 ha of fresh tomatoes under greenhouses. Cultivars resistant to Fusarium oxysporum f.sp. lycopersici (FOL) races 1 and 2 are mainly used. However, in 2012 and 2013, Fusarium wilt incidence of 2-3% was recorded. Symptoms appeared unilaterally and consisted of yellowing, leaf wilting of lower leaves, dark brown vascular discoloration, and plant death. A study was conducted to determine the causal agent of tomato wilt in seven tomato greenhouses in the Azapa Valley. Based on morphological observations, PCR assays and pathogenicity tests, two causal agents were identified, namely FOL race 3 and F. oxysporum f.sp. radicis-lycopersici (FORL). FOL and FORL were reported earlier in other tomato growing areas of Chile, located over 1000 km south of the Azapa Valley. This is thought to be the first report of FOL race 3 and FORL in the Azapa Valley and FOL race 3 in Chile.