Analysis of genetic variation in Pinus species using SDS-page.
Genetic diversity and phylogenetic characterization among five Pinus species (wallichiana, roxburghii, brutia, strobus and elliottii) were investigated in the present study. Wallichiana, roxburghii and brutia are native while strobus and elliottii are exotic in nature. Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE) was used to explore phylogenetic characterization based on of Seed storage protein. The genetic distances were estimated among the 70 germplasms accessions, ranges from 0-100%. Using Popgen version 3.2, a total of 182 alleles were scored and Phylogenetic relationships were clustered in 2 main groups "A" and "B" comprising 36 and 34 accessions, respectively. At this end it is concluded that the sample size should be extended for better understanding of the phylogenetic relationship among these species.