First report of Diplodia pinea on Pseudotsuga menziesii in Turkey.
Diplodia pinea [Sphaeropsis sapinea] is a latent, opportunistic pathogen of Pinus and other coniferous species, including Pseudotsuga menziesii. The fungus causes twig blight, branch cankers, tree and seedling collar rot, root rot, and can also infect cones. D. pinea has often been reported causing tip and shoot blight on various Pinus spp. in different parts of Turkey. During disease surveys on Pinus spp. carried out in May 2012 in Izmit in the Marmara Region, typical shoot blight symptoms of D. pinea infection were also observed on the neighbouring P. menziesii trees. Shoots and cones of P. menziesii were investigated for the presence of D. pinea pycnidia. Based on morphological characteristics of the conidia and by PCR of the rDNA ITS region, the pathogen was confirmed as D. pinea. This is thought to be the first report of P. menziesii as a host of D. pinea in Turkey.