First molecular detection of Croatian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR).
The potato cyst nematodes (PCN), Globodera rostochiensis and Globodera pallida are economically important pests of the potato (Solanum tuberosum L. subsp. tuberosum) and are recognized as quarantine pests internationally. In Croatia, PCN were discovered for the first time in Međimurska county in 2001. Since then all identifications of PCN populations for monitoring purposes have been based on morphological characters. In order to introduce the use of the precise and reliable molecular method of PCN identification, the polymerase chain reaction (PCR) was conducted, for the first time in Croatia. Another aim of this investigation was to find out the composition of PCN populations in Croatia. For this purpose ten PCN populations from three Croatian counties (Međimurska, Varaždinska, Primorsko-goranska) were analysed. All populations in investigation were identified as G. rostochiensis.