In vitro regeneration and mass multiplication of Prunella vulgaris L.
This is the first report on the development of an in vitro regeneration protocol for the medicinally important plant Prunella vulgaris L. growing in Doda region of Jammu and Kashmir. A simple, high-frequency and reproducible protocol has been standardized. Callus induction from different explants was obtained on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ) and 2,4-dichlorophenoxy acetic acid (2,4-D) in various concentrations. However, the best response towards callus formation with internode explants was observed in the medium having 1.0 mg l-1 TDZ and 0.5 mg l-1 2,4-D. The callus thus obtained proliferated on MS medium containing 1.0 mg l-1 TDZ and 0.05 mg l-1 indole-3-acetic acid (IAA). The green nodular callus led to the formation of shoots on MS medium supplemented with 1.5 mg l-1 benzyl amino purine (BAP) and 0.01 mg l-1 IAA. The shoots from the nodal explants were observed with 0.5 mg l-1 BAP and 0.05 mg l-1 IAA on MS medium. The best rooting response was observed with 0.5 mg l-1 IBA. The regenerated plants were transferred to the field after acclimatization.