Detection of Fusarium oxysporum f. sp. dianthi races in Iran.
In 2011, an extensive sampling was conducted in carnation greenhouses in two Iranian provinces (Tehran and Markazi). Fungal isolates were identified as F. oxysporum based on morphological characters. In pathogenicity tests, all inoculated plants developed wilt symptoms after 50 days of incubation at 28-30°C. Fifty isolates were selected randomly for further molecular characterization by comparison with eight F. oxysporum f.sp. dianthi isolates from Italy. F. oxysporum f.sp. dianthi races were identified by PCR amplification of transposon insertions. Of the 50 isolates, 42 were classified under race 2 and two isolates were classified under race 1 (or 8). This is thought to be the first report of the detection of F. oxysporum f.sp. dianthi races in Iran.