Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

16S rRNA-base analysis of bacteroidetes and firmicutes diversity in the microbial flora of the red-eared slider (Trachemys scripta elegans) intestinal tract.

Abstract

The intestinal tract of a vertebrate colonizes many microbial communities. In the normal intestinal flora, bacteroidetes and firmicutes as the dominant microbial, play an important role in host physiology health. In order to better understand the invasive species red-eared slider intestinal microflora of bacteroidetes and firmicutes diversity, we extracted the metagenomic DNA and analyzed the fecal microflora of 3 adult individuals using the Roche 454 sequencing technology. Fecal bacteria were characterized by amplifying the V3-V5 region of bacterial 16S rRNA gene. The sequencing result showed that bacteroidetes were identified as 2 classes, 5 families and 12 genera, bacteroidia (98.82±1.24)% was the absolute dominant microflora; firmicutes were identified as 3 classes, 11 families and 38 genera, mainly by Clostridia (95.81±0.88)%. In addition, a large number of unclassified sequences presented (4.38±1.64)% in bacteroidia and (14.96±8.15)% in firmicutes. The results of this study demonstrate that bacteroidetes and firmicutes have abundant diversity in fecal samples of the different red-eared slider. Roche 454 sequencing analysis based on 16S rRNA gene can reveal the composition of intestinal microflora.