First report of Botryosphaeria dothidea causing white rot of apple fruit in Serbia.
In December 2010, fruits of apple cv. Idared with symptoms of white rot were collected from storage in Svilajnac, Serbia. The incidence of the disease was low but symptoms were severe. Affected fruits were brown, soft, and almost completely decayed, while the internal decayed tissue appeared watery and brown. A fungus was isolated from the symptomatic tissue of one fruit. Fungal colonies were initially whitish, but started turning dark grey to black after 5-6 days. Pycnidia produced after 20-25 days contained one-celled, elliptical, hyaline conidia. These morphological characteristics were similar with those described for B. dothidea. Genomic DNA was isolated from the fungus and internal transcribed spacer (ITS) region of rDNA was amplified with the primers ITS1/ITS4 and sequenced. The nucleotide sequence has been assigned to GenBank Accession No. KC994640. BLAST analysis of the 528-bp segment showed a 100% similarity with several sequences of B. dothidea deposited in NCBI GenBank. This is thought to be the first report of B. dothidea on apple fruits in Serbia.