First report of Grapevine yellow speckle viroid-1 and Hop stunt viroid infecting grapevines (Vitis vinifera) in India.
From March to July 2012, around 10-15% of the plants of Vitis vinifera cultivars Thompson Seedless and Anab-e-Shahi exhibited yellow leaf spots and flecks, shortened internodes, and tiny yellow leaves in vineyards of the Bijapur, Doddaballapur, and Kolar districts of Karnataka State, India. Samples were collected from four plants from each district (12 samples in total) and RNA was extracted. The presence of Grapevine yellow speckle viroid 1 (GYSVd-1) was tested by reverse transcription (RT)-PCR for the amplification of a 220-bp region of the genome. In agarose gel electrophoresis, five samples showed amplicons of the expected size. These amplicons were cloned and sequenced. BLAST analysis confirmed the presence of GYSVd-1. All samples also tested positive for Hop stunt viroid in two separate sets of RT-PCR. This is thought to be the first report of GYSVd-1 and Hop stunt viroid infecting V. vinifera in India.