Identification of Poinsettia mosaic virus associated with a virus-like mottle symptom on poinsettia in Ohio.
In autumn of 2010 in Ohio (USA), several poinsettia samples were found exhibiting a virus-like mottle symptom. Results of ELISA using commercially available antibodies, PCR assay and BLASTn searches of the NCBI database demonstrate that the virus-like mottle symptom observed on poinsettia can be associated with Poinsettia mosaic virus (PnMV) infection. Both the degenerate tymovirus primers and the PnMV specific primers amplified products whose nucleotide and predicted amino acid sequences confirmed the amplicons were from PnMV. The PnMVfwd264 + rev1098 primer pair also amplified a second low MW product. That product was cloned and sequenced and was found to be a 187 bp fragment corresponding to the 3' end of the full length PnMVfwd264 + rev1098 amplicon, indicating the 5' primer is sufficiently complementary to a second site with the annealing temperature used for cycling. These results represent the first confirmed report of PnMV in poinsettia in Ohio.