First report of Impatiens necrotic spot virus in Mexico in tomatillo and pepper plants.
During the growing seasons of 2009 to 2011, surveys were conducted in the counties of Guanajuato and Querétaro in the states of the same names in Mexico. Sampling included tomatillo (Physalis ixocarpa) and pepper (Capsicum spp.) plantations where plants with possible viral symptoms were observed. The symptoms observed were dark necrotic spots on some leaves and on the stems. Leaf spots further developed into localized necrotic areas. ELISA with polyclonal antibodies, reverse transcription-polymerase chain reaction assay, and pathogenicity tests with Capsicum annuum cv. Cannon and watermelon (Citrullus lanatus), the causal agent was identified as Impatiens necrotic spot virus (INSV). This is thought to be the first report of INSV in Mexico found naturally in tomatillo and pepper and experimentally in watermelon plants.