First report of Impatiens necrotic spot virus on begonia in Bosnia and Herzegovina.
During a survey for the presence of Tospoviruses in July 2012, potted begonia hybrid (Begonia tuberhybrida) exhibiting foliar chlorotic rings and zonal spots accompanied by leaf necrosis and distortion were observed in a greenhouse in Banja Luka, Bosnia and Herzegovina. Leaf samples collected from 12 symptomatic plants were analysed for the presence of Impatiens necrotic spot virus (INSV) and Tomato spotted wilt virus (TSWV) by commercial double-antibody sandwich (DAS)-ELISA. INSV was detected serologically in all 12 begonia samples. All samples were negative for TSWV. For further confirmation of INSV infection, total RNAs were extracted from all ELISA-positive begonia plants and mechanically inoculated Nicotiana benthamiana plants and used as template in reverse transcription (RT)-PCR. The begonia isolate from Bosnia and Herzegovina showed the greatest nucleotide identity of 99.7% (100% amino acid identity) with the Chinese INSV isolate (FN400772) From Oncidium species. This is thought to be the first report of INSV on begonia in Bosnia and Herzegovina.