First report of tomato wilt caused by Ralstonia solanacearum biovar 1 in Puerto Rico.
In 2010 bacterial wilt was detected in tomato (Solanum lycopersicum) at a commercial farm in Coamo. Wilting of the foliage was observed in approximately 3,000 plants grown in a steep field. Bacterial streaming from the stem and an immunodiagnostic assay conducted in situ indicated that the disease was caused by Ralstonia solanacearum. The sequencing results of the siolates showed 100% homology with Ralstonia solanacearum. The sequences of isolates 1287-9 (Gen Bank Accession number JQ771188), 1287-6 (Gen Bank Accession number JQ771190) and 1290-2 (Gen Bank Accession number JQ771189) correspond to an upstream region genomic sequence of Ralstonia solanacearum. Isolates were identified as biovar 1. To prove Koch's postulates, forty 'Beefsteak' tomato seedlings were grown in a commercial substrate (PROMIX). Tomato plants were inoculated with isolates 1287-9 and 1290-2 with 1 ml of a suspension (10-8 CFU/ml of isolates 1287-9 and 1290-2) by injecting the base of the stem. A negative control with sterile water was included. Seven days after inoculation tomato plants developed flaccid leaves and wilted. This is thought to be the first report of Ralstonia solanacearum biovar 1 affecting tomato in Puerto Rico.