Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Development and application of the multiplex PCR method for the detection of Staphylococcus aureus, E. coli O157, Proteus vulgaris and Vibrio parahaemolyticus.

Abstract

Objective: To establish a multiple PCR system, being able to detect four kinds of food-borne pathogens quickly and accurately. It consists of Staphylococcus aureus, E. coli O157, Proteus vulgaris and Vibrio parahaemolyticus. Methods: Four primer were designed according to the target DNA sequences from above four kinds of pathogens. A multiplex PCR system was developed which can successful amplify four bacteria at the same time. Results: The results showed this multiplex PCR could effectively amplify the corresponding gene fragments. The sensitivity of the pathogens could reach 103 CFU/ml. Conclusion: The established multiplex PCR method exhibit more special, rapid, practical in detecting the above four pathogenic bacteria in foods.