Isolation and identification of a strain of porcine reovirus serotype 1 in China.
In order to study the molecular characteristics of porcine reovirus, a strain of reovirus, SHR-A, was isolated from Vero cell line by the method of adding trypsin into the culture DMEM from piglets' diarrhea fecal sample. The stable cytopathic effect caused by the virus including cell granule increasing, cellular swelling and shedding were observed. SHR-A strain was purified based on reovirus physical and chemical properties, and identified by conventional virological methods. Sequence analysis showed that the strain belonged to reovirus serotype 1. S1 gene (1 465 bp in full-length) of SHR-A showed higher similarity to that of mammalian orthoreovirus(MRV) serotype 1 than that of other serotype viruses. This is the first isolate of reovirus serotype 1 from porcine herds in China. 3′-UTR of SHR-A S1 genome segment shared higher homology to that of serotype 3 than to serotype 1, indicating that the S1 gene was a chimera of serotype 1 and serotype 3. The results provided evidence that MRV as RNA virus might have undertaken genomic recombination in the process of viral evolution, which is the third process beside genomic reassortment and genomic point mutation.