First report of Grapevine leafroll-associated virus 1 infecting grapevines in India.
A survey was conducted during 2010 and 2011 in the Nashik and Pune regions of western India (Maharashtra), and reddening of interveinal areas and downward rolling, typical symptoms of leafroll disease in dark fruited cultivars, were observed, first in 2010 and subsequently in 2011. Fourteen leafroll symptomatic samples from 7 cultivars of 7 vineyards were collected during 2011. GLRaV-1 was detected in two cultivars, Shiraj (Nashik region) and Pinot Noir (Pune region), using DAS-ELISA. GLRaV-1 was detected either alone in Pinot Noir or as mixed infection with GLRaV-3 in Shiraj. The presence of the virus in the two cultivars was confirmed by reverse transcription-PCR assay. This study represents the first report of GLRaV-1 from India.