First report of Meloidogyne marylandi infecting bermudagrass in Florida.
Using morphometric analysis and an RFLP method, an investigation of a golf course green in Florida with a history of infestation by root-knot nematodes was conducted to identify the species present. This 'Tifdwarf' bermudagrass (Cynodon dactylon × C. transvaalensis) putting green at the University of Florida Research Unit in Citra, FL, exhibited irregular patches of declining turf. Turf roots in these symptomatic areas had galles root tips with adhering egg masses, characteristics of infection from Meloidogyne spp. DNA was extracted from 15 single juveniles and sequences were compared with those in GenBank by means of BLAST search. The comparison showed a sequence similarity of 98% with M. marylandi (GenBank Accession No. JN241918.1). This is thought to be the first detection of M. marylandi in Florida.