Identification of Brome mosaic virus in cocksfoot (Dactylis glomerata L.) and meadow fescue (Festuca pratensis Huds.) in Lithuania.
Brome mosaic virus (BMV) causing viral diseases in graminaceous plants worldwide has been isolated in Lithuania from common cocksfoot (Dactylis glomerata L.) and meadow fescue (Festuca pratensis Huds.) plants exhibiting mosaic, chlorotic mottling and streaks of leaf and stem symptoms. The plant material was collected in the fields and roadsides of Vilnius and Kaunas regions. Virus isolates were investigated by test-plants, serology and reverse transcription-polymerase chain reaction (RT-PCR) methods. The identification of the virus was based on the results of symptomology on host-plants, transmission of viral infection by mechanical inoculation on test-plants, positive double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) test and a specific amplification fragment size (450 bp) of virus RNA in RT-PCR. This is the first report of common cocksfoot and meadow fescue as a natural host for BMV in Lithuania.