First report of lesion nematode (Pratylenchus vulnus) on boxwood in Ohio.
Boxwood (Buxus sempervirens and other species) is a popular evergreen shrub used in landscaping. In January 2012, three nursery-grown plants of boxwood cv. Green Gem were submitted from Warren County, Ohio, to the C. Wayne Ellet Plant and Pest Diagnostic Clinic at The Ohio State University. The plants, established for 4 years, exhibited orange to bronze discoloration of the foliage; foliage was not desiccated and dieback was not evident although stunning was present. Plant root symptoms ranged from nearly complete necrosis to distinct black lesions on living roots. A root scraping showed nematodes present in the lesions. The nematode was identified as P. vulnus based on morphological and morphometric characteristics, which was confirmed by PCR assays. This is thought to be the first report of P. vulnus in Ohio.