First report of Plum bark necrosis stem pitting-associated virus in stone fruit trees in Tunisia.
During a survey carried out in 2 stone fruit mother blocks located at Kairouan (central Tunisia) and Jendouba (northern Tunisia), a total of 212 leaf samples were collected from peach (70), almond (37), apricot (53), plum (36) and cherry (16) trees, respectively. These samples were tested by DAS-ELISA for the presence of Plum bark necrosis stem pitting-associated virus (PBNSPaV). Serological analysis showed that nearly 8% of the samples were infected by PBNSPaV, particularly those from peach (15.7%), plum (11.1%) and almond (5.4%) trees. Virus presence was confirmed by RT-PCR. This is thought to be the first report of PBNSPaV from Tunisia.