Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of Phoma herbarum on Tedera (Bituminaria bituminosa var. albomarginata) in Australia.

Abstract

A leaf spot symptom involving pale brown lesions with distinct dark brown margins had been observed in genetic evaluation plots of tedera (Bituminaria bituminosa) at Medina and Mount Barker, Western Australia, and a Phoma sp. was isolated. Amplification of the internal transcribed spacer (ITS) 1 and ITS2 regions flanking the 5.8S rRNA gene were conducted with universal primers ITS1 and ITS4. The DNA PCR products were sequenced and BLAST analyses was used to compare sequences with those in GenBank. The sequence had 99% nucleotide identity with the corresponding sequence in GenBank for P. herbarum. Isolates showed morphological and molecular similarities with P. herbarum. The relevant sequence information for a representative isolate was deposited in GenBank (Accession No. JQ282910). This is thought to be the first report of P. herbarum as a pathogen of tedera.