Detection of Zucchini yellow mosaic virus transmission through seeds of Siraitia grosvenorii.
In order to understand whether the Zucchini yellow mosaic virus (ZYMV) transmitted through seeds of Siraitia grosvenorii. A total of 700 seeds were collected from ZYMV-infected plants. Four hundred of 700 seeds were cultivated directly in net-house for control pest, and 300 were cultivated in MS medium after surface sterilization with alcohol. ZYMV of young plantlets were detected by observation or RT-PCR with ZYMV-specific primers. Two hundred and thirteen young plantlets were survived from 700 seeds. No ZYMV-positive sample was detected by observed or RT-PCR with ZYMV-specific primers. The results indicated that ZYMV could not be transmitted via seeds of Siraitia grosvenorii, or the transmission rate was very low.