Invasive Species Compendium

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Abstract

Detection of Zucchini yellow mosaic virus transmission through seeds of Siraitia grosvenorii.

Abstract

In order to understand whether the Zucchini yellow mosaic virus (ZYMV) transmitted through seeds of Siraitia grosvenorii. A total of 700 seeds were collected from ZYMV-infected plants. Four hundred of 700 seeds were cultivated directly in net-house for control pest, and 300 were cultivated in MS medium after surface sterilization with alcohol. ZYMV of young plantlets were detected by observation or RT-PCR with ZYMV-specific primers. Two hundred and thirteen young plantlets were survived from 700 seeds. No ZYMV-positive sample was detected by observed or RT-PCR with ZYMV-specific primers. The results indicated that ZYMV could not be transmitted via seeds of Siraitia grosvenorii, or the transmission rate was very low.