Study on the anti-inflammatory effect of total flavonoids from Opuntia monacantha (Willd.) Haw.
To investigate the anti-inflammatory effect of total flavonoids from Opuntia monacantha (Willd.) Haw, total flavonoids was extracted with two different methods including water extraction alcohol precipitation method (WEAP) and ultrasonic wave-alcohol extraction method (UWAE). The anti-inflammatory effect was investigated through three models including acetic acid-induced peritoneal capillary permeability in mice, cotton-pellet granuloma of the mice and egg white-induced rat paw oedema. In result high-dosage of total flavonoids prepared from WEAP and UWAE methods had both significant inhibitory effects on penetration of capillary vessel in mice (P<0.01). Moreover, compared with dexamethasone sodium phosphate, high-dosage of WEAP had a better anti-inflammatory effect. Different doses of total flavonoids prepared from WEAP and UWAE methods also significantly decreased the proliferation of granuloma caused by implantation cotton pellet in mice (P<0.01), and anti-inflammatory effect of the high-dosage of WEAP and low-dosage of UWAE was similar to dexamethasone sodium phosphate. Nonetheless, total flavonoids prepared by UWAE method was associated with excessive toxicity and was not suitable for medical use. High-dosage of total flavonoids prepared with WEAP method exhibited good attenuation effects on rat paw edema induced by egg white (P<0.01), with its anti-inflammatory effect similar to dexamethasone sodium phosphate. In conclusion, total flavonoids from Opuntia monacantha (Willd.) Haw has remarkable anti-inflammatory effects in the animal models of both acute and chronic inflammation.