Studies on seed mycoflora of wheat (Triticum aestivum L.) treated with potassium nitrate and its effect on germination during storage.
The main aim of the present study was to enumerate the fungal species and their effect on germination associated with wheat seeds. Seeds of two cultivars viz., Kundan and HUW-234 of wheat (Triticum aestivum L.) were collected after harvesting from agriculture farm, Banaras Hindu University, Varanasi. These seeds were treated with potassium nitrate and examine for seed mycoflora by agar plate method and blotter method. Total sixteen fungal species were isolated from test cultivars by the standard techniques. Fungi isolated and identified were Alternaria alternata, Alternaria solani, Aspergillus candidus, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Curvularia lunata, Fusarium roseum, Fusarium semitectum, Penicillium citrinum, Penicillium rubrum, Rhizopus stolonifer, Trichoderma harzianum, Dark sterile mycelium and White sterile mycelium. During isolation, the blotter method yielded the higher number of fungi as compared to agar plate method. Germination of infested wheat seeds was determined by three methods viz., blotter method, multi-pot tray method and plastic pot method. Germination was decreased during storage period because fresh seeds showed better germination percent i.e., from 95 to 100% than stored seeds. Nitrate treated seeds show better germination percent than untreated seeds of both cultivars.