Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

First report of natural infection of Mungbean yellow mosaic india virus in two wild species of Vigna.

Abstract

During the rainy season of 2010, accessions of wild species of V. hainiana (IC-331615) and V. trilobata (IC-331436), grown at the Indian Institute of Pulses Research, Kanpur, Uttar Prradesh, India, showed yellowing of inter-veinal tissue and bright yellow spots in the leaves. All plants of V. hainiana (8 plants) and V. trilobata (15 plants) were affected. The disease severity measured in terms of percentage foliage yellowing was 80% in the former and 30% in the latter. Whiteflies (Bemisia tabaci) were observed feeding on plants of these accessions. The type of symptoms and presence of whitefly suggested the involvement of a begomovirus. The association of a begomovirus was confirmed by PCR using primer pairs specific to Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV). Rolling circle amplification and restriction digestion were conducted to further confirm the presence of DNA A and DNA B of MYMIV. Agarose gel electrophoresis revealed the presence of ∼2.7 kb DNA bands, further confirming the presence of MYMIV with both DNA A and DNA B components. This is thought to be the first report of MYMIV infecting V. hainiana and V. trilobata.