Invasive Species Compendium

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Abstract

First report of Tobacco mild green mosaic virus infecting tomato in Iran.

Abstract

From August 2009 to July 2010, symptoms of interveinal chlorosis and malformations and necrosis of leaves and stems were observed in commercial tomato fields in the Karaj district of Tehran, Iran. Affected plants dried up at the final stage of the disease. Sap inoculation in tobacco cv. Samsun from symptom-bearing leaves of tomatoes resulted in the development of systemic mosaic and top necrosis in young leaves. Total RNA was extracted from symptomatic leaf tissues and analysed by RT-PCR with Tobacco mild green mosaic virus (TMGMV) coat protein specific primers. The 750 bp TMGMV-specific PCR fragment was amplified from 6 tomato samples collected from 3 fields in Karaj district and from all the post-inoculated tobacco. No amplification was observed from healthy samples of tomato or non-inoculated tobacco leaves. The TMGMV amplicon was purified and directly sequenced. The resultant partial cp gene sequence was deposited in GenBank (Accession No. HQ593622). BLAST of the TMGMV sequence analysis showed 91-100% identity at the amino acid level and 72-80% identity at the nucleotide level, with those of TMGMV isolates in GenBank (EF469769.1, M34077.1, AJ429096.1 and AF103782.1). This is thought to be the first report of TMGMV in Iran.