Invasive Species Compendium

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Abstract Full Text

Pathogenicity of two strains of Penicillium griseofulvum.

Abstract

In 2008, postharvest rot was observed on apple (cv. Golden Delicious) fruits from Aosta and Lagnasco (Cuneo), Italy, after a storage period of 6-8 months at 1.0°C. The infected fruits showed brown spots, and a soft aqueous phase of rapid expansion at 20° C under highly humid conditions. The surface of the lesion had blue-green masses of spores. The margin between the decaying and the healthy part of the fruit was quite distinct. The morphological identification of the fungus (P. griseofulvum) was confirmed by PCR. The DNA sequences of the isolate were deposited in the GenBank, with accession numbers HQ012498 (for the strain from Valle dacute˜Aosta) and HQ012499 (for the strain from Piedmont). The strains were evaluated for pathogenicity on 4 cultivars of apple (Golden Delicious, Red Chief, Granny Smith and Royal Gala). Both strains were virulent on all cultivars at room temperature and at cold storage, although the growth of the fungus was more slow at 24±1°C. Patulin production was very high, averaging approximately 40-45 mg/kg. This is thought to be the first report of P. griseofulvum as a producer of patulin on apple in Italy.