Characterization of Maize chlorotic mottle Virus associated with maize lethal necrosis disease in China.
A maize lethal necrosis disease was observed in Yunnan province of China. Isometric virus particles 30 nm in diameter were found in infected maize leaf tissues. Using DAS-ELISA, diseased maize plant samples reacted positively with the antiserum of Maize chlorotic mottle virus (MCMV). The complete nucleotide sequence (4436 nt) of a Yunnan isolate of MCMV was determined; it shares 97% nucleotide sequence identity with previously reported MCMV isolates. This is the first report of MCMV occurring in China.