First report of Botrytis hyacinthi on pineapple lily in California.
In late November 2007, foliage of pineapple lily (Eucomis vandermerwei) was submitted to the California State Diagnostic Laboratory by a commercial grower for phytosanitary inspection before shipment of bulbs to Europe. Leaves had wet, brown, elliptical spots that were 1-2 cm long, some with yellow halos. The interior of the older spots was pale brown and papery. Conidiophores typical of Botrytis spp. were observed on the abaxial side of the leaf in the interior of the older spots. Isolations on half-strength acidified potato dextrose agar yielded a greyish mycelium with conidiophores developing on the plant tissue only. The agar was greyish yellow, especially when viewed from the underside. Conidiophores measured 275-650 × 15-20 µm. Conidia were light brown, sub-globose to broadly ellipsoidal, and measuring 10-15 × 13-20 µm. Scattered, black sclerotia measuring 335-1007 × 518-1079 µm formed on the medium after approximately 7 days. The sequence of the intergenic spacer regions of one isolate, GenBank FJ10809, matched B. hyacinthi sequence AJ716297 with 99.8% identity. This is thought to be the first report of B. hyacinthi causing a leaf spot on pineapple lily.