Invasive Species Compendium

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Abstract

Cloning and sequence analysis of a ribosomal protein S11 gene in the oriental armyworm, Mythimna separata (Walker).

Abstract

Objective: The ribosomal protein S11 (RPS11) gene in M. separata was cloned and characterized in order to provide scientific data for further investigation on ribosomal protein function in insects. Method: The full-length cDNA of RPS11 gene was cloned using RT-PCR and RACE technique; the sequence of full-length cDNA and the deduced protein were analysed using bioinformatics, and the phylogenetic trees of RPSH was constructed. Result: The full-length cDNA of RPS11 gene in M. separata was 521 base pairs (bp) and contained a 5′-untranslated region (5′-UTR) of 26 bp and a 3′-untranslated region (3′-UTR) of 36 bp. The open reading frame of 459 bp encoded a 152 amino acid protein which shared typical ribosomal protein S17 family signature and 97% identity with Heliothis virescens RPS11. The phylogenetic tree constructed by the neighbour-joining method based on the amino acid sequences of M. separata RPS11 and other organisms showed that the relationships among M. separata, H. virescens, Spodoptera frugiperda and Bombyx mori were closer. Conclusion: The full-length cDNA sequence of RPS11 gene was obtained successfully in M. separata in this study (GenBank accession no. GQ222274), and the deduced protein based on the cDNA sequence of RPS11 gene belongs to ribosomal protein S17 family.