Bacterial wilt of solanaceae caused by Ralstonia solanacearum race 1 biovar 3 in Mali.
Isolates (25) of Ralstonia solanacearum were obtained from wilting potato, pepper, aubergine, tobacco, and tomato plants collected from fields near Baguineda, Sonityeni, Sotuba, Sikasso, and Kolikoro, Mali. Isolations were made from bacterial streaming by dilution plating on triphenyl tetrazolium chloride medium (TZC). Characteristic colonies were selected and identified by ELISA or Immunostrips. These isolates were used in host range studies and hypersensitivity (HR) tests on tobacco (cv. xanthi) and tested for their ability to produce acids on Ayers basal media amended with disaccharide and hexose alcohol carbon sources. Results indicate that all isolates were race 1, biovar 3. isolates. This is thought to be the first time that the race and biovar of R. solanacearum from Mali has been reported and the wide distribution of this pathogen in Malian soils and surface water has been demonstrated.