Toxicological and phytochemical studies of wild plant Halocnemon strobilacium crude extracts and their components against Aphis Craccivora Koch.
Toxicological and phytochemical studies were conducted on the Halocnemon strobilacium. Results indicated that the ethyl acetate crude extract with LC50 value 0.159 mg/cm2 and LC95 value 1.845 50 mg/cm2 was the most effective against Aphis craccivora comparing with fraction B (LC50 1.522 mg/cm2 and LC95 4.721 mg/cm2) as well as fraction A (LC50 1.299 mg/cm2 and LC95 3.899 mg/cm2). Data also showed that, the crude extract was composed mainly of saturated and unsaturated fatty acids and their esters (69.34%) followed by hydrocarbons (9.89%). Only two terpenes were identified in this crude extract, one of them was Phytol isomer, a diterpene detected with 2.26%. Oleic acid ester (38.18%) and Octadecanoic acid (16.44%) were the major constituents. The main compounds in Fraction A were hydrocarbons, fatty acids and their esters. Lumiflavine represented about 35.66. Five fatty acids and esters were identified and constituted 39% with Oleic acid ester (14.74%) Octadecanoic acid methyl ester (4.41%) and Tetradecanoic acid (3.88%) being the major representatives. In fraction B twenty three compounds were identified. Unoxygenated compounds amount to 9.89, the main hydrocarbons being dimethyl undecane (5.20%) and trimethyldecane (1.01%). Oxygenated compounds constitute 90.11% of the fraction comprising fatty acids and esters, ketones as well as aldehydes (46.08%, 17.94% and 3.29% of the fraction respectively). The main ester identified was octadecanoic acid, methyl ester (3.44%) while hexadecanoic acid was the main fatty acid detected (22.69%).