Detection of Bordetella avium by polymerase chain reaction in the lungs and tracheas of turkeys with pneumonia.
Lung, trachea, and serum samples obtained from turkeys showing clinical signs of respiratory disease were examined for the presence of Bordetella avium infection (bordetellosis) using culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). The material of this study consisted of 2 live turkeys at the age of 24 weeks with respiratory distress, nasal discharge, and cough that had been brought to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Adnan Menderes University in Turkey. The results showed that B. avium, the causative agent of bordetellosis, was not isolated from either the lung or trachea, while samples from the trachea and lung were positive in PCR. The presence of antibodies against B. avium in all serum samples was detected in ELISA. However, sera samples were negative in PCR. In conclusion, it was thought that ELISA is a practical assay although the results of ELISA and PCR were found complementary. This was the first study in Turkey that reported the detection of B. avium in turkeys in using the PCR. However, further studies should be planned with more materials to understand the epidemiological importance of the disease in poultry in this region.