Invasive Species Compendium

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Abstract

Simultaneous detection of six RNA plant viruses affecting tomato crops using a single digoxigenin-labelled polyprobe.

Abstract

A polyprobe for the simultaneous detection by non-isotopic molecular hybridisation has been developed to detect any of the following six viruses causing important economic losses in tomato crops: Tomato spotted wilt virus, Tomato mosaic virus, Pepino mosaic virus, Cucumber mosaic virus, Potato Y virus and Parietaria mottle virus. The polyprobe detected all six viruses with similar sensitivity to that obtained using individual riboprobes. In addition, we evaluated the possible use of the tissue-printing as a sample preparation technique applied to routine diagnosis of tomato plants with the polyprobe. Electronic supplementary material The online version of this article (doi:10.1007/s10658-008-9347-5) contains supplementary material, which is available to authorized users.