Invasive Species Compendium

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Abstract

Identification of muskmelon gummy stem blight pathogen and its biological characters.

Abstract

Stems of musk melon infected with gummy stem blight were collected from Jiangsu province, China. The morphological and biological characters of the 4 strains of pathogenic fungi isolated from the sampled stems were investigated. The effects of temperature (10, 15, 20, 25 or 30°C) and pH (5.0, 5.5, 6.0, 6.5, 7.0, 7.5 or 8.0) on the performance of the isolates were studied. The optimum temperature for fungal growth was 25°C. The pH value of the media had no significant effect on the growth of the pathogen. The rDNA-ITS genes of the 4 isolates were amplified and sequenced. Based on BLAST search and GenBank database search, along with the study of the morphological characters of the isolates, the pathogen causing musk melon gummy stem blight was identified as Didymella bryoniae (Ascochyta citrullina as the anamorph).