Detection of seed borne fungi of some selected medicinal plants.
An experiment was conducted in the Seed Health Laboratory, Department of Plant Pathology of Sher-e-Bangla Agricultural University, Dhaka Bangladesh during from January to June 2006. Occurrence of fungi in seeds of ten selected medicinal plants Andrographis paniculata, angel's trumpet (Datura metel), Asparagus racemosus, butterfly pea (Clitoria ternatea), changeable rose (Hibiscus mutabilis), devil's cotton (Abroma augusta), Indigofera tinctoria, Jatropha panduraefolia, liquorice (Glycyrrhiza glabra) and Margosa tree (Melia azadirachta [Azadirachta indica]) were detected. Inspection of dry seeds revealed that the seed samples contained apparently healthy seeds, discoloured seeds, and shrivelled and malformed seeds. The germination percentage was recorded higher in changeable rose (88.50%) and lower in Margosa tree (41.00%) in blotter method. Germination percentage was recorded higher in blotter method than pot soil test. Fungi were detected by blotter method both with and without pretreatment of seeds. HgCl2 (0.01%) solution was used for pretreatment. Seven fungal species, Alternaria tenuis [Alternaria alternata], Aspergillus flavus, Aspergillus niger, Curvularia lunata [Cochliobolus lunatus], Fusarium oxysporum, Penicillium sp. and Rhizopus stolonifer, were recorded from without pretreated seeds while only three species of fungi, Alternaria tenuis, Curvularia lunata and Fusarium oxysporum, were recorded from pretreated seeds. All these fungi are new records for seeds of medicinal plants in Bangladesh.