RAPD analysis on genetic relationships among Sphaeropsis sapinea isolates.
Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea collected from Pinus radiata, P. elliottii, P. banksiana, P. nigra, P. massoniana, P, sylvestris, P. resinosa, P. taeda, P. caribaea, P. pinaster, P. griffithii [P. wallichiana] and Cedrus deodara, in China, the USA, England, South Africa and Chile by using RAPD analysis method. 135 scorable DNA fragments were amplified with twelve random primers by polymerase chain reaction technique and the polymorphic score was 96.3%. The genetic dendrogram based on RAPD analysis showed that all S. sapinea isolates could be clustered into three groups. The isolate CWS41 from Chile was genetically separated as the first group, and isolates F2 and J2 from China were the second group. The third RAPD group accommodated the other isolates (including B morphotype CWS43 from the USA).