Detection of Bean yellow mosaic virus (BYMV) infecting Gladiolus cultivars collected from different sources.
The presence of bean yellow mosaic virus (BYMV) was evaluated in Gladiolus cultivars collected from different sources in New Delhi, India: Sylvia, Pusa Sugahan, American Beauty and Sancerre from Horticultural Garden IARI; Happiness, Legend, Melody and Bright Yellow from Division of Biochemistry IARI; Mayur, Suchitra and Ave from Sunder Nursery, Hauz Khas; Carnival and Oscar from Sheetal Nursery, Mehrauli; and Gold Dust and Spectacular from Delhi University. Evaluation was done via DC-ELISA using antiserum to BYMV isolate, and by leaf dip preparation in EM. Strong reactions were observed in Mayur, followed by Sylvia, Ave and Suchitra. Very mild reactions were observed in Pusa Sugahan, American Beauty, Sancerre, Carnival, Oscar, Happiness, Gold Dust, Legend, Melody and Spectacular. No reaction was observed in Bright Yellow.